We’ve been on a long series about Methylation and how an individual’s nutrition can affect the outcome of their health. Here’s a quick review of MTHFR:
Once the MTHFR tests shows (+) variants exist, what else can the health provider do to effectively treat the patient?
So, what are you doing as a patient to take control of your health in relation to genome testing and restorative care with a physician that understands the impact of these aspects?
As a medical practitioner, are you having your patients tested for this all important genome so that they can be more proactive with their health and prevent disease?
Children with autism had significantly lower baseline plasma concentrations of methionine, SAM, homocysteine, cystathionine, cysteine, and total glutathione and significantly higher concentrations of SAH, adenosine, and oxidized glutathione.
Consistent with impaired capacity for methylation (significantly lower ratio of SAM to SAH) and increased oxidative stress (significantly lower redox ratio of reduced glutathione to oxidized glutathione).
Metabolic biomarkers of increased oxidative stress and impaired methylation capacity in children with Autism 1 From the Department of Pediatrics, University of Arkansas for Medical Sciences, and the Arkansas Children‘s Hospital Research Institute, Little Rock, AR (SJJ, SM, and SJ); Niagara Falls, NY (PC); Colden, NY (LJ); Gaylor and Associates, LLC, Eureka Springs, AR (DWG); and Edison, NJ (JAN)2 Rep
- 6 years old
- HM hx of severe anxiety,
- unable to perform in class,
- small bumps on cheeks and upper arms mom describes as acne..
The mother of the child took him to the doctor to medicate him for anxiety and for a referral to a special education program. The mother was considering home schooling due to the severity of the child’s anxiety. This testing was done in addition:
Labs: HLA Dq2/ Hla Dq8 negative
Food allergy test + gluten, wheat, dairy, honey, yeast, and some other minor antibodies (leaky gut).
Stay tuned for the results on our next blog.
A xenobiotic is a foreign chemical substance found within an organism that is not normally, naturally produced by or expected to be present within that organism. It can also cover substances which are present in much higher concentrations than are usual.
In humans, 2-Oh and 4-Ohestradiol (catechol estrogens) are rapidly O-methylated to form monomethyl ethers catalyzed by COMT and S-adenosyl-L-methionine.
Xenobiotics may strongly inhibit COMT-mediated
O-methylation of catechol estrogens by xenobiotics and may facilitate the development of estrogen-induced tumors.
Xenobiotics may therefore deplete intermediates in the Folate cycle. Environmental burden of Xenobiotics may create a higher need for methylation support.
Abstract: COMT genotype, micronutrients in the folate metabolic pathway and breast cancer risk - Goodman JE, Lavigne JA, Wu K, Helzlsouer KJ, Strickland PT, Selhub J, Yager JD; Department of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, MD 21205, USA.
Catechol-O-methyltransferase (COMT) catalyzes the O-methylation of catechol estrogens (CEs), using S-adenosylmethionine (SAM) as a methyl donor.
Several studies have indicated that the val108met COMT polymorphism, which results in a 3-4-fold decrease in activity, is associated with increased breast cancer risk.
Folate, whose intake levels have also been associated with breast cancer risk, and other micronutrients in the folate metabolic pathway influence levels of SAM and S-adenosylhomocysteine (SAH), a COMT inhibitor generated by the demethylation of SAM.
Because these micronutrients have been shown to alter SAM and SAH levels, we hypothesized that they could also affect COMT-catalyzed CE methylation.
Although measurements of SAM and SAH were not initially collected, a secondary analysis of data from two nested case-control studies was performed to examine whether serum levels of folate, vitamin B12 (B12), pyridoxal 5′-phosphate (PLP), cysteine and homocysteine, in conjunction with COMT genotype, were associated with breast cancer risk. COMT(HH) (high activity COMT homozygote) breast cancer cases had statistically significantly lower levels of homocysteine (P = 0.05) and cysteine (P = 0.04) and higher levels of PLP (P = 0.02) than COMT(HH) controls. In contrast, COMT(LL) (low activity COMT homozygote) cases had higher levels of homocysteine than COMT(LL) controls (P = 0.05).
No associations were seen between B12, COMT genotype, and breast cancer risk. An increasing number of COMT(L) alleles was significantly associated with increased breast cancer risk in women with below median levels of folate (P(trend) = 0.05) or above median levels of homocysteine (P(trend) = 0.02). These findings are consistent with a role for certain folate pathway micronutrients in mediating the association between COMT genotype and breast cancer risk.
These findings are consistent with a role for certain folate pathway micronutrients in mediating the association between COMT genotype and breast cancer risk.
- Treatment: 28 day detox, gluten, diary, soy free, with protein smoothie (rice) due to low body weight.
- Di-indole methane and 3 indole-carbinol for estrogen balance, NAC,(glutathione)
- Trimethyglycine/Methyl -folate & B12 for methylation support
- L-theanne/Gaba, 5-HTP to improve sleep and pain control
- Co-Q10 support
- Selenium/Lipoic acid,vit E for anti-oxidant support
- High concentrate EPA/DHA Magnesium Glycinate 200mg qhs for alkaline, mineral, and bowel support
- Probiotics, 50 bill/ FOS
- Exercise daily, check urine ph goal> 6.6. Sauna at the gym, alkaline diet, Xenoestrogen awareness!
Susan’s response: “I feel the best that I have felt in years. This is miraculous! I sleep deeper than ever, there are no hot flashes, I’m exercising because I have ENERGY, and my husband says I’m back again because I’m SASSY again!”
49 yo CF recent dx of Stage II Breast Ca hormone Rec +,s/p partial mastectomy, radiation, irregular periods, shingles, intolerant of Fereston due to Headaches, constipated. Hx of fibroids, heavy periods, fibrocystic breast. G2P2
nl vitals, Estradiol 26.8pg/ml (0-32.2 menopause range)
Sed rate 22 (0-20)
Cbc, chem, thyroid panel cholesterol wnl
Vit D 153 (30-100)
GGT 15 Uric Acid 3.2
Cytokine: base Il-6++, IL-17+, IL-12–,IFN gamma ++, TNF-a ++, IL-4++, IL-5–, IL-10++, IL-8++, G-CSF++
How would you approach treatment of this patient?
- Involved in phase II metabolism of hydroxy-estradiols
- Involved in metabolism of xenobiotics
- Involved in metabolism of chocolate
- Involved in metabolism of caffeine by-products (catechins)
- Involved in metabolism of excitatory neurotransmitters
- S-adenosylmethionine and magnesium dependent
- Linked to estrogen
- imbalance disorders
- Is involved in hyperhomocystinemia in Parkinson patients on L-dopa
- Is linked to psychiatric disorders
This may lead to megaloblastic anemia as seen in elevated MCV in the patients CBC.
Patients may actually have high levels of B12 in the serum as the Cobalamine is not converting to Methylcobalamine.
May compound mutations in MTHFR
Supplementation with megadoses of MeB12 has been advocated to protect the cognitive function of patients suffering from:
Alzheimer’s disease Neurological diseases
- Transsulfuration is the conversion of Homocysteine with the co-factors B6 and Serine to Cystathionine.
- Cystathionine is then converted to Cysteine which is one of the three amino acids that compose Glutathione.
- Polymorphisms in Cysta beta-synthase may affect the rate of conversion of Cystathionine to Cysteine.
- Cysteine is thought to be the rate limiting step in the formation of Glutathione.
- chronic low glutathione
- leaky gut and food allergies
- chronic viruses
- high serum B12 levels
- low serotonin and melatonin
- high serum ferritin and HFE mutation
- Thalassemia and hepatorenal failure
- FSG and renal failure
- autoimmunity frequency in women
- chronic dysbiosis/chronic yeast infections
- estrogen dominant cancers
- subtype and elevated homocysteine
- ADD/bipolar/PMS/Chronic Fatigue/Fibromylagia
- tongue/fingernails/thin hair
- vericose veins/spider veins
- elevated testosterone in men/PCOS in women
- acne, fibroids, Endometriosis, Menometorrhagia
- depression when on OCPs
- exacerbation of menopausal symptoms on BHRT
- elevated uric acid and elevated ammonia levels
- gut barrier and dysbiosis
- estrogen dominance and obesity (estrogens effects on insulin and the insulin receptor and adiponectin)